Fluorescent Assay Protocols & Demonstrations
Based on demonstrations we have conducted, as well as work we have done with current customers, we have put together three different types of documents.
Assay Overview
These documents provide a quick snapshot of how an assay could be performed on the Celigo, with some images and results of the assay. Download the complete Celigo Assay Overview eBook for all Assay Overviews.
Demonstration Experiment
These documents are created from actual experiments that have been conducted on the Celigo. The documents review how the experiment was done using the Celigo, and summarizes the results from the experiment. Download the complete Celigo Demonstration Experiment eBook for all Demonstration Experiments.
Application Protocol
These documents are instructional, and provide step-by-step details for customers to follow if they are running the assay on their Celigo, or for potential customers to review if they are setting up the experiments they will be conducting during the Celigo demonstration. For a copy of an Application Protocol document you see listed available here, please reach out to your local Applications Scientist, email us at support@nexcelom.com or call 978-327-5340
| Title | Assay Overview | Demo Experiment | Application Protocol |
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| Cell cycle analysis using DAPI and BrdU |  |
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| Cell viability analysis using calcein AM, Hoechst and PI | |
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| Endpoint apoptosis using Caspase 3/7 with Hoechst | |
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| Cell cycle analysis using EdU and DAPI | |
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| Endpoint viability using DRAQ7 and Hoechst | |
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| Kinetic viability using DRAQ7 | |
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| High-throughput suspension cell count and viability using AO/PI | |
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| High-throughput suspension cell count and viability using AO/PI and BF | |||
| p53 and phosphor-p53 fluorescent marker analysis | |
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| Endpoint viability using Propidium Iodide and Hoechst | |
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| Kinetic viability using PI | |
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| Kinetic apoptosis using Caspase 3/7 | |
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| Cell Cycle PI Analysis using A375 cells | |||
| HPC proliferation measurement using Ki-67 cellular marker | |
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| Antibody-Dependent Receptor Internalization Assay | |
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| Count and measure cell infectivity of microcarriers | |
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| Antibody Cell Surface Binding Detection | |
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| GFP Transfection Efficiency Measurement | |
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| Antibody-Dependent Drug Uptake Assay | |
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| Kinetic pHrodo Antibody Internalization using Confluence | |
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| Kinetic antibody internalization using pHrodo and bright field | |
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| Endpoint antibody internalization using pHrodo and Hoechst | |