Measure Migration from 3D Spheroid onto ECM

Measure the Cell Outgrowth from a Spheroid onto an Extra Cellular Matrix (ECM)

  1. Directly image tumor spheroids in various microwell formats
  2. Non-invasive bright field imaging allows the user to image the same plate over multiple days
  3. Perform a two-color fluorescent viability assay
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Celigo Product Guide
Applications Collection

Introduction

The Celigo imaging cytometer has been developed to fully automate imaging and analysis of tumorspheres. This automated morphometric analysis tool significantly reduces the time and effort needed to quantify key aspects of 3D spheres including size, growth, growth tracking over time and response to chemotherapeutics.

automate imaging and analysis of tumorspheres

Measured Outgrowth from a HUVEC 3D Tumor Spheroid

Spheroid

Image on hour 0 of spheroid outgrowth

Bright field image

Spheroid

Image on hour 0 of spheroid outgrowth

Bright field image

Monitoring Cell Outgrowth from a GFP-labeled HUVEC Spheroid

Image of monitored cell outgrowth from GFP-labeled spheroid

Spheroid

Spheroid image showing cell outgrowth 1

Bright field image

Spheroid fill view

Spheroid image showing outgrowth fill view

Bright field image

Tumorspheroid image showing outgrowth 2
Spheroid image showing cell outgrowth 3
Spheroid image showing cell outgrowth 4